ParkinsonCanadaV1, France, Analysis, bibRecord, 000278

Peptidase activities of the 20/26S proteasome and a novel protease in human brain

Identifieur interne : 000278 ( France/Analysis ); précédent : 000277; suivant : 000279

Peptidase activities of the 20/26S proteasome and a novel protease in human brain

Auteurs : Sophie Vigouroux [France] ; Yoshiaki Furukawa [Canada] ; Luc Farout [France] ; Stephen J. Kish [Canada] ; Marièle Briand [France] ; Yves Briand [France]

Source :

Journal of neurochemistry [ 0022-3042 ] ; 2003.

RBID : Pascal:03-0245854

Descripteurs français

Pascal (Inist)
- Peptidases, Multicatalytic endopeptidase complex, Omptin, Ubiquitine, Expression génique, Activité enzymatique, Démence Alzheimer, Parkinson maladie, Homme.
Wicri :
- topic : Homme.

English descriptors

KwdEn :
- Alzheimer disease, Enzymatic activity, Gene expression, Human, Multicatalytic endopeptidase complex, Omptin, Parkinson disease, Peptidases, Ubiquitin.

Abstract

Many neurodegenerative diseases are characterized by ubiquitin-positive protein aggregates or inclusion bodies. Ubiquitin-conjugated proteins are degraded by the 20/26S proteasome, and reduced proteasome peptidase activities in brain homogenates have been reported in pathologic lesions of Parkinson's and Alzheimer's diseases. However, it is unknown whether crude extracts of human brain contain other proteases having peptidase activities. We found a novel protease of molecular weight of approximately 105 kDa in normal human brain, which exhibited trypsin-like (T-L) and chymotrypsin-like (ChT-L) activities (corresponding to 52% and 21% of the total activities in crude extracts) but not peptidyl glutamyl peptide hydrolase activity. Both T-L and ChT-L activities of this protease were partially inhibited by proteasome inhibitors (MG132, lactacystin) and, in contrast to those of the proteasome, also by sodium dodecyl sulfate. A simple method to obtain a brain fraction specific to the 20/26S proteasome was developed. Our human brain data suggest that T-L and ChT-L activity levels of the proteasome reported previously may include those of the 105 kDa protease, an enzyme of as yet unknown biological significance, and that it is necessary to separate the proteasome from this protease to evaluate the actual status of the ubiquitin-proteasome system in neurodegenerative disorders.

Affiliations:

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Pascal:03-0245854

Le document en format XML

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<front><div type="abstract" xml:lang="en">Many neurodegenerative diseases are characterized by ubiquitin-positive protein aggregates or inclusion bodies. Ubiquitin-conjugated proteins are degraded by the 20/26S proteasome, and reduced proteasome peptidase activities in brain homogenates have been reported in pathologic lesions of Parkinson's and Alzheimer's diseases. However, it is unknown whether crude extracts of human brain contain other proteases having peptidase activities. We found a novel protease of molecular weight of approximately 105 kDa in normal human brain, which exhibited trypsin-like (T-L) and chymotrypsin-like (ChT-L) activities (corresponding to 52% and 21% of the total activities in crude extracts) but not peptidyl glutamyl peptide hydrolase activity. Both T-L and ChT-L activities of this protease were partially inhibited by proteasome inhibitors (MG132, lactacystin) and, in contrast to those of the proteasome, also by sodium dodecyl sulfate. A simple method to obtain a brain fraction specific to the 20/26S proteasome was developed. Our human brain data suggest that T-L and ChT-L activity levels of the proteasome reported previously may include those of the 105 kDa protease, an enzyme of as yet unknown biological significance, and that it is necessary to separate the proteasome from this protease to evaluate the actual status of the ubiquitin-proteasome system in neurodegenerative disorders.</div>
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	La maladie de Parkinson au Canada (serveur d'exploration)
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La maladie de Parkinson au Canada (serveur d'exploration)

Peptidase activities of the 20/26S proteasome and a novel protease in human brain

Peptidase activities of the 20/26S proteasome and a novel protease in human brain

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri